Figure 8. Vps8 overexpression strongly decreases Vps41 binding to the class C core.
Endogenous Dor/Vps18, Vps16A and Car/Vps33A class C proteins coprecipitate with Vps41-9xHA isoforms (marked by PA and PC, respectively) from larval lysates. The amount of coprecipitated class C proteins decreases to less than 9% in lysates of larvae overexpressing Vps8. Asterisk marks a nonspecific band. Numbers under the bands in IP lanes refer to class C protein levels normalized to Vps41-9xHA PA+PC based on densitometry.