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. 2019 Jun 12;8:e47102. doi: 10.7554/eLife.47102

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© 2019, Box et al

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Figure 1. — (A) Schematic of E14.5 skin depicting several planar cell polarity components and example division orientations. Dotted line represents focal plane for live imaging. (B,C) Stills from time-lapse movies of Vangl2^WT E14.5 skin explants expressing K14-H2B-GFP, showing examples of planar (B) and perpendicular (C) division orientations. Top panels are the planar view of the basal layer of the epidermis, bottom panels are XZ dimension. Scale bar = 10 μm. See also Figure 1—video 1 and Figure 1—video 2. (D) Example and quantification of division angles in live epidermal explants at E14.5. Vangl2^WT, n = 310 divisions pooled from three embryos. Vangl2^Lp/Lp, n = 271 divisions from three embryos. Modified Kuiper’s Test, p=1.7523e-15. (E) Distribution of division orientations in live epidermal explants at E14.5. Planar: Θ <= 20°, unpaired two-tailed t-test p=0.027; oblique: 20°>Θ>=70°, p=0.030; perpendicular: 70°>Θ>=90°, p=0.427. n = 3 explants from each genotype. (F) Angular frequency of division angles quantified from fixed whole mount skins dissected from embryos e13.5 – e16.5. E13.5: Vangl2^WT, n = 208 divisions from three embryos; Vangl2^Lp/Lp, n = 198 divisions from two embryos; p=2.8648e-08. E14.5: Vangl2^WT, n = 164 divisions from three embryos; Vangl2^Lp/Lp, n = 166 divisions from three embryos; p=1.5118e-18. E15.5: Vangl2^WT, n = 152 divisions from three embryos; Vangl2^Lp/Lp, n = 116 divisions from three embryos; p=0.3482. E16.5: Vangl2^WT, n = 132 divisions from three embryos; Vangl2^Lp/Lp, n = 115 divisions from three embryos; p=0.1785.