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. 2019 Jun 25;8:e46045. doi: 10.7554/eLife.46045

Figure 1. Two cell populations expressing both T cell (CD3) and monocyte (CD14) surface markers exist in the live singlet cell population of PBMC from human subjects.

(A) The top 100 most variable genes in memory CD4+ T cells across TB uninfected (TBneg) and LTBI infected subjects. (B) Immune cell type specific expression of the 22-var genes identified in A). Every bar consists of stacked sub-bars showing the TPM normalized expression of every gene in corresponding cell type. Expression of genes for the blood cell types shown were taken from the DICE database (Schmiedel et al., 2018, http://dice-database.org/). (C) Detection of CD14+ events within sorted CD4+ memory T cells and (D) non-parametric spearman correlation between their frequency and the PC1 from the 22-var genes. (E) Gated on ‘singlet total live cells’, two populations of CD3+CD14+ cells can be identified based on the level of expression of CD14. (F) Based on FSC and SSC parameters, CD3+CD14hi cells are contained within the monocyte gate, whereas CD3+CD14mid cells are contained within the lymphocyte gate. Data were derived from 30 LTBI subjects and 29 TB uninfected control subjects.

Figure 1—source data 1. Non-parametric spearman correlation between the 22-var genes in memory CD4+ T cells from human subjects.
Memory CD4+ T cells were sorted from 30 LTBI subjects and 29 TB uninfected control subjects (gating strategy Figure 1—figure supplement 1A) and their transcriptomic profile defined by RNA sequencing. The 22-var genes were identified as presented in Figure 1A.
elife-46045-fig1-data1.xlsx (13.6KB, xlsx)
DOI: 10.7554/eLife.46045.005

Figure 1.

Figure 1—figure supplement 1. Identification of a CD14 +population within memory CD4 T cells.

Figure 1—figure supplement 1.

(A) Gating strategy to isolate bulk memory CD4+ T cells. (B) Backgating of CD14+ cells within sorted memory CD4+ T cells.
Figure 1—figure supplement 2. Gating strategy to identify CD3+CD14+ cells.

Figure 1—figure supplement 2.