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. 2019 Jun 25;8:e46045. doi: 10.7554/eLife.46045

Key resources table.

Reagent type (species) or resource Designation Source or reference Identifiers Additional information
Antibody CCR7-PerCpCy5.5; clone G043H7; mouse monoclonal Biolegend Cat# 353220 1:50 (4 µl per test)
Antibody CD2-BV421; clone RPA-2.10; mouse monoclonal Biolegend Cat# 300229 1:66 (3 µl per test)
Antibody CD3-AF700; clone UCHT1; mouse monoclonal BD pharmigen Cat# 557943 1:66 (3 µl per test)
Antibody CD3-AF488; clone UCHT1; mouse monoclonal Biolegend Cat# 300415 1:200 (1 µl per test)
Antibody CD4-APCeF780; clone RPA-T4; mouse monoclonal eBiosciences Cat# 47-0049-42 1:200 (1 µl per test)
Antibody CD5-APCCy7; clone L17F12; mouse monoclonal Biolegend Cat# 364009 1:66 (3 µl per test)
Antibody CD7-APC; clone CD7-6B7; mouse monoclonal Biolegend Cat# 343107 1:66 (3 µl per test)
Antibody CD8a-BV650; clone RPA-T8; mouse monoclonal Biolegend Cat# 301042 1:200 (1 µl per test)
Antibody CD14-APC; clone 61D3; mouse monoclonal Tonbo biosciences Cat# 20–0149 T100 1:200 (1 µl per test)
Antibody CD14-AF594; clone HCD14 Biolegend Cat# 325630 1:200 (1 µl per test)
Antibody CD14-AF647; clone 63D3 Biolegend Cat# 367128 1:200 (1 µl per test)
Antibody CD14-BV421; clone HCD14 Biolegend Cat# 325628 1:200 (1 µl per test)
Antibody CD14-PE; clone 61D3; mouse monoclonal eBioscience Cat# 12-0149-42 1:200 (1 µl per test)
Antibody CD27-BV650; clone O323 Biolegend Cat# 302827 1:100 (2 µl per test)
Antibody CD33-APC; clone WM53 Biolegend Cat# 303407 1:200 (1 µl per test)
Antibody CD36-APCCy7; clone 5–271 Biolegend Cat# 336213 1:100 (2 µl per test)
Antibody CD45-PerCpCy5.5; clone HI30; mouse monoclonal Tonbo biosciences Cat# 65–0459 T100 1:66 (3 µl per test)
Antibody CD45RA-eF450; clone HI100; mouse monoclonal eBiosciences Cat# 48-0458-42 1:200 (1 µl per test)
Antibody CD64-AF488; clone 10.1; mouse monoclonal Biolegend Cat# 305010 1:200 (1 µl per test)
Antibody CD163-PECy7; clone GHI/61; mouse monoclonal Biolegend Cat# 333613 1:100 (2 µl per test)
Antibody ICAM1(CD54); unconjugated; clone HCD54; mouse monoclonal Biolegend Cat# 322704 1:40 (5 µl per test)
Antibody LFA1(CD11a); unconjugated; clone TS2/4; mouse monoclonal Biolegend Cat# 350602 1:40 (5 µl per test)
Antibody LFA1(CD11a/CD18)-AF647; clone m24; mouse monoclonal Biolegend Cat# 363412 1:40 (5 µl per test)