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. 2019 Jun 20;12:4839–4848. doi: 10.2147/OTT.S192521

Figure 5.

Figure 5

Effects of WT1 upregulation on hsa-miR-1270-correlated GBM proliferation and migration in vitro.

Notes: (A) In LN-18 and A172 cells with stable hsa-miR-1270 upregulation, they were secondary transfected with pc/WT1 or pc/C. After transduction was stabilized, we applied quantitative real-time PCR to examine endogenous WT1 expressions in secondarily transfected LN-18 and A172 cells (*P<0.05). (B) In an in vitro proliferation assay, secondary-transfected LN-18 and A172 cells were allowed to grow for five consecutive days. Their proliferation rates were measured and compared at an absorbance of 570 nm (*P<0.05). (C) In an in vitro migration assay, secondary-transfected LN-18 and A172 cells were given 24 hours to migrate in a Transwell plate. GBM cells attached to the bottom of Transwell plate were stained by 0.1% crystal violet and imaged. (D) For imaging result in (C), in hsa-miR-1270-upregulated L-18 and A172 cells, cancer migration was compared between pc/WT1- and pc/C-transfected cells (*P<0.05).

Abbreviations: GBM, glioblastoma multiforme; WT, wild type; WT1, Wilms’ tumor gene.