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. Author manuscript; available in PMC: 2019 Jun 25.
Published in final edited form as: Nature. 2017 Sep 27;550(7674):124–127. doi: 10.1038/nature24039

Figure 4. ZAP binds directly and preferentially to CG dinucleotide-containing RNA.

Figure 4

a, CLIP analysis of the frequency with which L mutant and HIV-1WT RNA sequences are bound to ZAP in infected cells, versus their position in the viral genome. CG dinucelotides are indicated as blue lines. The L-mutant segment occupies positions 6307 to 6805. b, Expanded views of the ‘L’ portion of the CLIP graphs in a. c, Frequency distributions of CG, GC, UA and UG dinucleotide observed/expected frequencies in human ORFs, 3′UTRs and the top 100 ZAP-binding sites. P-values for ZAP binding sites (n=100) versus ORFs (n=35170) or 3′UTRs (n=135557) calculated using Welch’s unequal variance t-tests.