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. Author manuscript; available in PMC: 2019 Jun 25.
Published in final edited form as: Nature. 2017 Sep 27;550(7674):124–127. doi: 10.1038/nature24039

Extended data Figure 4. smFISH quantification of total HIV-1 RNA in infected cells.

Extended data Figure 4

Examples of smFISH analysis of WT and synonymous mutant HIV-1 infected cells (red=smFISH probe targeting all viral mRNA species (see Fig 2c), green=GFP, blue=Hoescht dye) Clusters of RNA molecules in the nuclei of some infected cells may represent sites of proviral integration. Representative of 3 independent experiments. Scale bar = 5μm.