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. Author manuscript; available in PMC: 2020 Jul 1.
Published in final edited form as: J Am Soc Mass Spectrom. 2019 Apr 9;30(7):1276–1283. doi: 10.1007/s13361-019-02192-9

Figure 4.

Figure 4.

Heat map for differentially regulated selenoproteins in macrophages post LPS treatment. Briefly, BMDMs were cultured with 100 ng/ml LPS for 20 hours. The samples were processed as described under “Methods”. Panel A shows upregulation of proto l proteins upregulated in response to LPS. Panel B shows temporal modulation of selenoproteins in macrophages. The color scale bar represents protein relative abundance, red (low) to blue (high). Panel C & D show representative (of n= 3) Western immunoblot validation of Selenow, Gpxl, and Txnrdl and densitometric values in bar graphs. NS- Nonspecific immunoreactive band. Two-way ANOVA multiple comparisons *, p<0.05; **, p<0.009; ***, p<0.0009; ****, p<0.0001. All experimental data are expressed as mean ± S.E.