Table 1.

Selenoproteins identified by TMT labeling and analyzed on Orbitrap Tribrid Fusion MS. Selenoproteins can be divided into two groups depending on the Se position in amino-acid sequence. In first group, the Sec position is towards N-terminal or in the middle region, the other group have towards C-terminal end. Selenoproteins identified in macrophages by TMT 10plex labelling and corresponding TMT labelled peptide position is provided in Figure 2 and Figure S2.

Sl	Uniprot Accession	Protein name	Gene Symbol	#AAs	Sec position	TMT-LBUP	#PSMs
Sec in the N-terminal or middle region
1	P11352	Glutathione peroxidase 1	Gpx1	201	47	9	91
2	O70325	Phospholipid hydroperoxide glutathione peroxidase	Gpx4	197	73	10	72
3	Q9ERR7	Selenoprotein F	Selenof	162	93	4	56
4	Q8VHC3	Selenoprotein M	Selenom	145	48	1	2
5	D3Z2R5	Selenoprotein N	Selenon	557	428	2	3
6	P62342	Thioredoxin reductase-like selenoprotein T	Selenot	195	49	2	19
7	P63300	Selenoprotein W	Selenow	88	13	1	2
Sec in the C-terminal region
8	Q9JMH6	Thioredoxin reductase 1, cytoplasmic	Txnrd1	613	612	22	199
9	Q9JLT4	Thioredoxin reductase 2, mitochondrial	Txnrd2	524	523	9	23
10	Q9JLC3	Methionine-R-sulfoxide reductase B1	Msrb1	116	95	1	1
11	Q80TA1	Ethanolaminephosphotransferase 1	Selenoi	398	388	1	2
12	Q9JLJ1	Selenoprotein K	Selenok	94	92	2	3
13	Q9BCZ4	Selenoprotein S	Selenos	190	189	3	6