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. Author manuscript; available in PMC: 2019 Jun 25.
Published in final edited form as: J Mol Med (Berl). 2015 Mar 11;93(8):891–904. doi: 10.1007/s00109-015-1264-4

Fig. 4. Renal morphology and functional parameters in adult mice with deletion of HIF-1α in EC.

Fig. 4

a H&E-stained kidney sections show no morphological difference in wild-type (Tie2-cre(−)) and endothelial HIF-1α knockout (Tie2-cre(+)) mice (×200, scale bar 100μm). b Immunofluorescent MECA32 stainings (×400) and quantification of renal sections show no differences in renal vessel density (scale bar 20 μm; n=5–7). c Renal function (plasma creatinine (upper panel) and urea (lower panel)) is not significantly different between genotypes. d Body weight (upper panel) and two kidney-to-body weight (bw) ratio (lower panel) as well as water intake (e) and urine production per 24 h (f) in metabolic cages are not affected by deletion of HIF-1α in EC in mice (for all metabolic measurements—WT, n=3; KO, n=6)