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. Author manuscript; available in PMC: 2019 Jun 25.
Published in final edited form as: Mol Microbiol. 2017 Feb 6;104(1):92–104. doi: 10.1111/mmi.13614

Fig. 5.

Fig. 5

The observed increased secretion is not mediated by cell lysis or outer membrane vesicles.

A. NCTC11168 grown in the presence of subinhibitory concentrations of novobiocin shows no significant effect on growth.

B. NCTC11168 grown overnight in the presence of novobiocin shows no significant difference in numbers of colony forming units.

C. NCTC11168 supernatant from bacteria grown in the presence of novobiocin does not react with antibodies raised against the outer membrane protein CadF.

D. NCTC11168 protein secreted when grown in the presence of novobiocin is not resistant to proteinase K treatment. 1-Whole cell lysate, 2-Whole cell lysate, Prot. K treated, 3-Whole cell lysate boiled, Prot. K treated, 4-Uninduced secreted fraction, 5-Uninduced secreted fraction, Prot. K treated, 6-Induced fraction, 7-Induced fraction, Prot. K treated.