DNA relaxation leads to increased protein found within the supernatants of multiple C. jejuni strains and novobiocin induced secretion is inhibited by mutations in the flagellar genes flgI, flgE and fliD.
A. Coomassie staining of concentrated bacterial supernatants from C. jejuni strains 11168, C19 and 81-176 grown in the absence and presence of novobiocin.
B. No increase in protein within supernatants or whole cell lysates (WC) of strain 81-176 containing mutations in genes flgI, flgE or fliD is observed following DNA relaxation.