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. 2019 Jun 25;9:9243. doi: 10.1038/s41598-019-45502-7

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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miR-20a inhibits autophagy activity during chondrogenic differentiation. (A) mRNA expression of miR-20a in eight groups after transfection of miRNA reagents, addition of an autophagy inhibitor or their combination under inducing culture. (B,C) Western blotting results of autophagy-related proteins, including LC3-II/I, p-Bcl-2, and p62, and the chondrogenic marker protein col2. Comparisons between each of the two groups were analysed using Dunnett’s test. (D,E) Immunofluorescent assay of col2 and quantification by ImageJ. *p < 0.05, **p < 0.01. *Without a connecting line is compared to the control group.