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. 2019 Jun 25;9:9208. doi: 10.1038/s41598-019-45519-y

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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LC-MS/MS analysis of metabolite production by recombinant E. coli. Samples corresponding to 50 µl of culture supernatants were analysed. (a) SPE-treated bacterial supernatants of cultures of BL21AI expressing CDPS74 and BrePT (blue), EchPT1 (pink), FtmPT1 (green), NotF (orange) or CDPS74 alone (black) were analysed. UV traces recorded at 220 nm are shown between 6 and 30 min with the absorbance scale set from 0 to 700 mU. Asterisks highlight specific peaks for which the MS data are indicated. (b) SPE-treated bacterial supernatants of cultures of BL21AI expressing CDPS68 and CdpC3PT (blue) or CDPS68 alone (pink) were analysed. UV chromatograms recorded at 220 nm (UV₂₂₀, plain lines) and extracted ion current at m/z 368 (EIC₃₆₈, dotted lines) are shown between 6 and 30 min. The Y-axis of the UV₂₂₀ traces was set from 0 to 700 mU and that of the EIC₃₆₈ traces from 0 to 3,380,000.