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. 2019 Jun 25;9:9224. doi: 10.1038/s41598-019-45661-7

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Cumulative frequency graph of variant numbers for chromosomes 27 (a) and 2 (b) in female and male chickens for genomic variant enrichment analysis. Chromosomes 2 and 27 (Fig. 2) were selected in order to draw the cumulative frequencies graph of variants along the chromosome. It is shown by Figure (a) that the most variants enrichments are available in the range of 2692–1 × 10⁶ base pairs (separated with horizontal line) of chromosome 27. A total of 234 variants was recognized on chromosome 27, and 100 (42.72%) variants were also located in the range of 2692–1 × 10⁶ region. Figure (b) indicates that 17891 variants were recognized on chromosome 2, but 7600 variants (43%) were located in the range of 41 × 10⁶–81 × 10⁶ (27% of chromosome length) base pairs. Finally, these regions with the highest density of variation were selected for the identification of candidate genes.