Fig. 3.

m⁶A enrichment in mRNA, and the function of m⁶A in melanoma cells. a, b m⁶A dot blot assays using total RNA of Mel624 and B16F10 cells with or without FTO knockdown. Methylene blue staining was used as a loading control. c, d m⁶A dot blot assays using poly(A) + mRNA of Mel624 and B16F10 cells with or without FTO knockdown. e Immunoblot analysis confirming forced overexpression of METTL3 and METTL14 in Mel624 cells. f m⁶A dot blot assays using poly(A) + mRNA in Mel624 with or without forced overexpression of METTL3 and METTL14. g Cell proliferation assay in Mel624 cells with or without forced overexpression of METTL3 and METTL14. h Cell migration assay in cells in G. i Cell invasion assay in cells in g. j–m Cell proliferation (j), migration (k), invasion (l), and cell viability (m) assays in suspension in shNC and shFTO of Mel624 with or without knockdown of METTL3 (M3) and METTL14 (M14). Data are shown as mean ± S.D. (n ≥ 3). *P < 0.05; **P < 0.01; ***P < 0.001; Student’s t-test