Fig. 4.

Identification of potential targets of FTO in melanoma. a Schematic summary for analysis of FTO target genes and number of genes identified. b Relative expression of FTO, PD-1 (PDCD1), CD274, and CD47 in Mel624 cells with or without FTO knockdown. c Distribution of m⁶A peaks across the length of mRNA. Region of 5-untranslated region (5′UTR) was binned into 10 segments, coding region (CDS) was binned into 50 segments, and 3-untranslated region (3′UTR) was binned into 40 segments, and the percentage of m⁶A peaks that fall within each bin was determined. d The proportion of m⁶A peak distribution in the indicated regions across the entire set of mRNA transcripts (top) and the appearance of new m⁶A peaks (unique peaks in shFTO), or loss of existing m⁶A peaks (unique peaks in shNC) after FTO knockdown (bottom). e Number of m⁶A-modified genes identified in m⁶A-seq in shNC and shFTO Mel624 cells. Common m⁶A genes contain at least one common m⁶A peak, while unique m⁶A genes contain no common m⁶A peaks. f Top consensus m⁶A motif identified by HOMER with m⁶A peaks in Mel624 cells with or without FTO knockdown. g qPCR analysis of mRNA levels of genes in Mel624 cells with shNC or shFTO. h Gene-specific m⁶A qPCR analysis of gene-specific m⁶A enrichment in Mel624 cells with shNC or shFTO. Data are shown as mean ± S.E. (n ≥ 3). *P < 0.05; **P < 0.01; ***P < 0.001; Student’s t-test