Figure 1.

Differential metabolites and unsupervised hierarchical clustering. Data sets resulting from the LC-MS/MS runs of inner ears of control and noise-exposed mice (after filtering out under-loaded samples) were subjected to unsupervised hierarchical clustering. The control and noise-exposed samples segregate according to exposure with the exception of a few samples. The suffixes of “A1” to “E5” in the sample names represent 3 biological replicate experiments with each LC method and mice numbers: A = experiment 1 with RPLC, B = experiment 2 with RPLC, C = experiment 2 with HILIC, D = experiment 3 with RPLC, and E = experiment 3 with HILIC.