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. 2019 Jun 25;9:9276. doi: 10.1038/s41598-019-45635-9

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Hypersynchronization in hippocampal CA3 pyramidal neurons network in Mecp2^−/y at P15 is rescued by bumetanide. (a) Representative traces of dual whole-cell voltage clamp recordings of glutamatergic repetitive population bursts recorded at −75 mV from two neighboring hippocampal CA3 pyramidal neurons in hippocampal slices from P15 Mecp2^−/y mice. Lower traces are shown at extended timescale. (b) Relative contribution of cells displaying population bursts in wt, Mecp2^−/y and Mecp2^−/y with MP bumetanide represented in %. (c) Representative cross-correlation between sEPSC traces recorded in both cells shown in (a). (d) Averaged Pearson’s coefficient for dual sEPSC traces. (e) Representative traces of sEPSCs from “bursty” hippocampal CA3 pyramidal neurons recorded at −75 mV in Mecp2^−/y mice with and without acute bumetanide treatment. (f) Averaged cumulative probabilities of charge, amplitude and duration of bursts in brain slices of Mecp2^−/y and Mecp2^−/y with acute bumetanide treatment. (g) Burst mean parameters: charge, amplitude, duration and charge density in Mecp2^−/y and in Mecp2^−/y + bumetanide. Data are presented as mean ± SEM. *p < 0.05; ***p < 0.001. (b) Significance was determined with the two-tailed Fisher’s exact test with Bonferroni correction. (d) Pearson coefficient in wt and Mecp2^−/y. (g) Datasets were analyzed with the paired sample two-tailed t-test. See Supplementary Table 11 for detailed statistics.