Table 1.
Primers for real-time quantitative PCR
| Gene name | Primer name | Primer sequences | Size of product (bp) |
|---|---|---|---|
| ALG9 (reference gene) | ALG9-F | GGACTGATTTCTATTTTGAGGATAATGAAC | 168 |
| ALG9-R | TATAGTTGTCGGGAAGGAAGAAAGATGCTGG | ||
| UBC6 (reference gene) | UBC6-F | ACACGTGGAATCCAGCCTGGAGTGT | 264 |
| UBC6-R | GCTGCTTTTTCTGCCGCCAATGC | ||
| HOG1 | HOG1-F | ATACATCCCACCGACCACAAG | 219 |
| HOG1-R | CCAGATGTTGCGTGGATTGAA | ||
| GPD1 | GPD1-F | CCAGCAGCAATAGCAACAACA | 245 |
| GPD1-R | TCCCAATCCATCACTGACACT | ||
| GPP1 | GPP1-F | ACCACAATATCAGCTCCGGAA | 160 |
| GPP1-R | CTCAAGGCTACAAATCGGCAC | ||
| GUT1 | GUT1-F | TGCTCAGCCACATTATGCCTT | 232 |
| GUT1-R | AGCCCTGGCAATATGACTAGA | ||
| GUT2 | GUT2-F | CCACAGCAGTAGCCTTGATGA | 186 |
| GUT2-R | TGAAAGCTGCATGTGTGTACC | ||
| FPS1 | FPS1-F | GCACCAAAGACCCCAGGAGGAAT | 96 |
| FPS1-R | CGGCAGTAGACAAAGCAGAAGCAAG |