FIGURE 3.

(A) Schematic representations of the AMER3 and SLC9A9 mRNAs depicting predicted binding sites (BS) for let-7f-5p in their 3′-UTR. (B) Levels of let-7f-5p 48 h after transfection with pre-let-7f or control scramble. Values are means ± S.E.M. from three separate experiments. ^∗P < 0.05 compared with cells transfected with control scramble. (C) Representative immunoblots of AMER3 and SLC9A9 in cells described in (B). (D,E) Overexpression of let-7f-5p significantly inhibited the WT but not the Mut luciferase activities of AMER3 and SLC9A9 in Caco2 cells. ^∗P < 0.05 compared with cells transfected with control scramble.