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. 2019 Jun 19;10:1385. doi: 10.3389/fmicb.2019.01385

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Copyright © 2019 Grabinger, Glaus Garzon, Hausmann, Geirnaert, Lacroix and Hennet.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Mono- and oligosaccharide utilization by bacterial cultures. B. acidifaciens, B. vulgatus, and R. gnavus were cultured in chopped meat medium (CMM) to a final OD₆₀₀ of 0.8. Then they were transferred to Hungate tubes with growth-limiting YCFA medium containing 20 mM of fucose (Fuc), glucose (Glc), 2-fucosyllactose (2FL), 3-fucosyllactose (3FL), or 10 mM of lactose (Lac) to an initial OD₆₀₀ of 0.05. Negative control tubes (CTRL) contained YCFA medium without any carbohydrate substrate. Growth was monitored until stationary phase was reached. Mean values are shown from triplicates of 2 independent growth experiments. Error bars represent SD.