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. 2019 Jun 19;9:484. doi: 10.3389/fonc.2019.00484

Figure 6.

Figure 6

Combination treatment Pre ALL cells with curcumin and cisplatin potentiated apoptotic effects. (A) Curcumin and cisplatin augmented annexin-V positive cells. SupB15 cells were treated with 10 μM curcumin and 10 μM cisplatin alone or in combination for 24 h as indicated. The cells were then stained with fluorescein-conjugated annexin-V, PI, and analyzed by flow cytometry. (B) The graph displays the mean ± SD (standard deviation) of the percentage of apoptotic cells three independent experiments with replicates of three wells for all the doses. *p < 0.001. (C) SupB15 cells were treated with 10 μM curcumin and 10 μM cisplatin alone or in combination for 24 h cells were lysed and 50 μg of proteins were separated on SDS-PAGE, transferred to PVDF membrane, and immunoblotted with antibodies against caspase-3, cleaved caspase-3, PARP, p-H2AX, and GAPDH.