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. 2019 Jun 19;10:793. doi: 10.3389/fpls.2019.00793

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Copyright © 2019 Miguel, Nisse, Biteau, Rottloff, Mignard, Gontier, Hehn and Bourgaud.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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RT-PCR exhibiting the capacity of TMV to infect and to move systemically into N. alata plant in 14 days. Use of specific primers to movement protein gene (502 bp); M, Marker; W, water; +, RNA from N. benthamiana infected by TMV; 1, 2, 3, RNA of different parts of N. alata plant inoculated with safety N. benthamiana extract; 4–6, RNA of different parts of N. alata plant inoculated with N. benthamiana extract infected by TMV; 1 and 4, RNA from inoculated leaf; 2 and 5, RNA from pitcher attached to inoculated leaf; 3 and 6, RNA from neighboring leaf. Parts of gels separated by a space were grouped together and lined up to facilitate the visualization of results. Any changes touching the quality of picture were applied to every pixel of each part.