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. 2019 Jun 19;10:793. doi: 10.3389/fpls.2019.00793

Figure 5.

Figure 5

RT-PCR exhibiting the capacity of recombinant TMV to infect and to move systemically into N. alata plant in 14 days. (A) Use of specific primers to TMV capsid protein gene (480 bp); (B) Use of specific primers to gfp (246 bp); M, Marker; W, water; +, RNA from N. benthamiana infected by TMV-wt (A) and agroinfiltrated by pMW388-gfp plasmid (B); 1 and 4, RNA from inoculated leaf with safety N. benthamiana extract at 5 days (1) and 14 days (4); 2 and 5, RNA from inoculated leaf with N. benthamiana extract infected by TMV-wt and agroinfiltrated by pMW388-gfp at 5 days (2) and 14 days (5); 3 and 6, RNA from pitcher attached to inoculated leaf with N. benthamiana extract infected by TMV-wt and agroinfiltrated by pMW388-gfp at 5 days (3) and 14 days (6) post inoculation. Parts of gels separated by a space were grouped together and lined up to facilitate the visualization of results. Any changes touching the quality of picture were applied to every pixel of each part.