Figure 1.

Disrupting DNA repair with Dbait molecules. Radiation and Dbait treatment induce DNA repair signaling disturbance. Dbait recognize and activates DNA-PK complex leading to its activation and its subsequent nuclear targets phosphorylation visualized by pan-nuclear γ-H2AX. When a DSB occurs in the DNA, the DNA damage signaling system activated by Dbait is spread across modified chromatin and prevents the arrival of proteins involved in DSB repair at site of the damage. Consequently, both non-homologous end joining (NHEJ) and homologous recombination (HR) were inhibited. Moreover, Dbait can also recognize PARP (mainly involved in BER and SSBR) causing its autoPARylation and leading to several BER and SSBR proteins recruitment on Dbait molecules. All these factors are thus hijacked far from the DNA damage site causing to BER/SSBR inhibition.