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. 2019 Jun 19;10:667. doi: 10.3389/fphar.2019.00667

Figure 2.

Figure 2

The LDN-193189 BMP inhibitor enhances metastases development in vivo. ZNF217-revLuc or pcDNA6-revLuc cells were delivered via intracardiac injection into the bloodstream of nude mice. (A) Kaplan–Meier analysis of metastasis-free mice in LDN-193189-treated (n = 8) and non-treated (n = 8) ZNF217-revLuc-injected mice. (B) Total metastases load measured by in vivo bioluminescence imaging in LDN-193189-treated (n = 8) and non-treated (n = 8) ZNF217-revLuc-injected mice. (C) Average number of metastases per ZNF217-revLuc-injected mouse. (D) Total bioluminescent signal per metastasis in LDN-193189-treated and non-treated ZNF217-revLuc-injected mice. (E) Kaplan–Meier analysis of metastasis-free mice in LDN-193189-treated (n = 8) and non-treated (n = 5) pcDNA6-revLuc-injected mice. (F) Total metastases load measured by in vivo bioluminescence imaging in LDN-193189-treated (n = 8) and non-treated (n = 5) pcDNA6-revLuc-injected mice. (G) Average number of metastases per pcDNA6-revLuc-injected mouse. Results are presented as mean ± standard error of the mean (SEM) in (B), (C), (D), (F), and (G). *P < 0.05 (Mann–Whitney test). (H) Distribution of the number of metastases developed by each group of mice.