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. 2019 Jun 25;9:9210. doi: 10.1038/s41598-019-45545-w

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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CRES amyloids are not cytotoxic to mammalian cells. Freshly prepared and aged mid- and high concentration CRES was added to mouse DC1 epididymal cells (0.17–17 μM final) in a 96 well plate and incubated for 20 hours at 37 °C, 5% CO₂ in media without serum. Freshly prepared Aβ_1–40 added to DC1 epididymal cells (0.2–2 μM final) served as a cytotoxic amyloid control. Cell viability was determined by MTT assay. Cells incubated with freshly prepared mid- and high concentration CRES showed similar MTT values and were pooled. C, control cells incubated with buffer only. T, cells incubated with 1% Triton x-100. All samples were done in duplicate. Data show the mean ± SEM of n = 3–6 biological replicates using 2–5 different CRES preparations. Statistical differences were determined by ANOVA followed by Tukey test. *p ≤ 0.05.