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. 2019 Jun 19;10:715. doi: 10.3389/fphys.2019.00715

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Copyright © 2019 Salvia, Grimaldi, Girardello, Scieuzo, Scala, Bufo, Vogel and Falabella.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Western blot analyses performed on exosome-like vesicles released from teratocytes stimulated with ATP. The incubation with anti-Ae-FABP (15 kDa) and anti-Ae-ENO antibodies (45 kDa) confirmed that both proteins are released by vesicles. The incubation with anti-HSP70 (70 kDa) and anti-ALIX (92 kDa) antibodies, specific exosomal markers, strongly supports the hypothesis that vesicles are exosomes.