Figure 4.

HEK293 Cells Expressing Gene-Edited NLuc-β₂-Adrenoceptor Labeled with HaloTag AF488 in the Absence or Presence of Transiently Transfected HaloTag-VEGFR2 (0.01 μg/well)

(A) Data from a single experiment performed with 36 replicates. *p < 0.05 (unpaired t test) compared with donor alone. Similar data were obtained in four separate experiments. In each repeat experiment significant differences (p < 0.05) were observed from donor alone apart from in one where p = 0.07.

(B) Mean paired data from the five separate experiments, each performed with 30 or 36 replicates. *p < 0.05 compared with donor only (paired two-tailed t test).

(C) Human umbilical vein endothelial cells (HUVECs) transiently transfected via electroporation with NLuc-VEGFR2 in the presence or absence of SNAP-β₂-AR. Data were pooled from eight independent transfections (four to eight replicates per experiment for each transfection) and expressed as mean ± SEM. *p < 0.05 compared with NLuc-VEGFR2 donor only (paired two-tailed t test; p = 0.035).