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. 2019 Jun 19;10:1344. doi: 10.3389/fmicb.2019.01344

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Copyright © 2019 Schumacher, Nicholson, Stoffel, Chandran, D’Mello, Ma, Vashee, Jores and Labroussaa.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Immunoblots performed after Triton X-114 phase partitioning showing the in vitro cytoplasmic localization of GlpO in the “Mycoplasma mycoides cluster.” Immunoblots were carried out using an anti-LppQ antibody (A), the anti-beta Galactosidase polyclonal serum (B) or the anti-glpO antibody (C–H) on total proteins of GM12::YCpMmyc1.1 (A,B), GM12::YCpMmyc1.1-ΔglpO (C), five different strains of M. mycoides subsp. capri (D), two strains of M. feriruminatoris (E), three strains of M. capricolum subsp. capricolum (F), two strains of M. leachii (G), three strains of M. mycoides subsp. mycoides (H), and one strain of M. capricolum subsp. capripneumoniae (I).