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. 2019 Jun 25;4(4):e00070-19. doi: 10.1128/mSystems.00070-19

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Copyright © 2019 Minato et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license.

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FIG 1 — Identification of essential genes in MtbYM rich medium. (A) Medium compositions of Mtbminimal and MtbYM rich media. 7H9 medium contains glutamic acid and ammonium sulfate in addition to the nutrients shown in bold. DAP, diaminopimelic acid; FAC, ferric ammonium citrate; PABA, para-aminobenzoic acid; *, 0.5% Casamino Acids and 0.98 mM tryptophan. (B) M. tuberculosis H37Rv growth in MtbYM rich (red), 7H9 (black), and Mtbminimal (blue) media. (C) Bayesian/Gumbel results for M. tuberculosis H37Rv in MtbYM rich medium. (D) HMM results for M. tuberculosis H37Rv in MtbYM rich medium. (E) Venn diagram comparisons of essential genes by Tn-seq in this study and two previous studies (8, 9). Red, this study; blue, Griffin et al. (8); green, DeJesus et al. (9).