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. 2019 Jun 19;10:1383. doi: 10.3389/fmicb.2019.01383

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Copyright © 2019 Lukumbuzya, Schmid, Pjevac and Daims.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Simultaneous visualization, by multicolor FISH, of seven microbial target groups in activated sludge. The target organisms, assigned false colors, rRNA-targeted oligonucleotide probes, and fluorochromes are indicated. (A) Combined maximum intensity projection of confocal image stacks of each probe signal. Scale bar, 20 μm. The size of the image stacks along the z-axis was 12 μm. For the purpose of illustration, the brightness of the projection was enhanced by using the respective tool of the daime software. (B) Enlarged part of the image as indicated by the frame in panel (A).