Table 2.
Spectral and molecular properties of the fluorophores used for labeling rRNA-targeted oligonucleotide probes.
| Fluorophore | λmax (excitation) | λmax (emission) | Detection window (nm)a | MWb (g/mol) |
|---|---|---|---|---|
| Atto 425c | 439 nm | 485 nm | 475–495 | 401 |
| Atto 490LS | 496 nm | 661 nm | 645–685 | 696 |
| Atto 532 | 532 nm | 552 nm | 545–565 | 765 |
| Atto 565 | 564 nm | 590 nm | 580–600 | 611 |
| Atto 594 | 603 nm | 626 nm | 620–640 | 1137 |
| Atto 633 | 630 nm | 651 nm | 650–670 | 652 |
| DY-681d | 691 nm | 708 nm | 700–720 | 737 |
| FLUOS | 492 nm | 517 nm | 510–530 | 473 |
| Cy3 | 554 nm | 668 nm | 550–570 | 767 |
| Cy5 | 649 nm | 666 nm | 660–670 | 754 |
aWavelength windows used for recording the emitted fluorescence. bMW, molecular weight. cFor Atto 425, an excitation wavelength of 405 nm was used. dFor DY-681, an excitation wavelength of 670 nm was used.