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Validation of hepatocyte PPARα knockout efficiency in mice. A: Real-time PCR analysis of hepatic Ppara mRNA levels in PparaΔHep mice and Pparafl/fl mice. B and C: Western blot analysis of heptic PPARα protein expression in PparaΔHep mice and Pparafl/fl mice and statistical graph. Data are expressed as means ± SD (A and C). n = 5 (A); n = 2 (B and C). ∗∗P < 0.01, ∗∗∗P < 0.001 versus Pparafl/fl.
