Figure 1.
Vasoactive intestinal peptide (VIP) treatment of human corneal endothelial cells accelerates corneal endothelial wound healing in vitro in a dose-dependent manner. A:In vitro wound healing of cultured human corneal endothelial cells with or without VIP (10−12, 10−9, 10−7, 10−6 mol/L). Representative images show the remaining wounded area (yellow) in VIP-treated mice and in the control group (Opti-MEM) 3, 6, 12, and 24 hours after incubation. B: Percentage of healed endothelial area compared with baseline in VIP-treated groups and in controls. Human corneal endothelial cells treated with 10−9, 10−7, and 10−6 mol/L VIP demonstrate significantly enhanced wound healing compared with the control group 12 and 24 hours after incubation compared with the control (P < 0.05, U-test). A dose-dependent trend was observed in the effect of VIP. Data are expressed as means ± SEM and data from one of two independent experiments are shown (B). n = 9. Original magnification, ×40.