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CTLs secrete functional exosomes soon after 3SI stimulation. Exosomes were purified from 24-h (D1E) or 72-h (D3E) supernatant of CTLs stimulated with 3SI (MHC I/N4+B7-1+IL-12). (A) Representative size distribution and electron microscopy of 3 experiments. (B) Western blot based on 10 μg protein of exosomes from 3 different experiments. (C) Naive OT-I cells were stimulated with low-affinity peptide T4 with or without D1E or D3E for 2 days before staining.
