Table 4.
Correlation coefficients for comparison of phenotype abundances produced by PICRUSt2 with binary phenotype and MetaCyc pipelines.
| Vitamin | AGP | HMP | MetaCyc pathway(s) |
|---|---|---|---|
| B1 | 0.62 | 0.88 | Superpathway of thiamin diphosphate biosynthesis I |
| B2 | 0.79 | 0.87 | Flavin biosynthesis I (bacteria and plants) |
| B3 | 0.84 | 0.89 | NAD biosynthesis I (from aspartate); NAD biosynthesis II (from tryptophan) |
| B5 | 0.86 | 0.94 | Pantothenate and coenzyme A biosynthesis I |
| B6 | 0.57 | 0.62 | Pyridoxal 5′-phosphate biosynthesis I |
| B7 | 0.82 | 0.76 | Biotin biosynthesis I; biotin biosynthesis II |
| B9 | 0.89 | 0.93 | Superpathway of tetrahydrofolate biosynthesis |
| B12∗ | – | – | Adenosylcobalamin biosynthesis II (late cobalt incorporation); adenosylcobalamin biosynthesis I (early cobalt insertion) |
| Q | 0.78 | 0.91 | preQ0 biosynthesis |
∗Both anaerobic (early cobalt insertion) and aerobic (late cobalt incorporation) pathways of B12 biosynthesis received very low or zero abundances in most analyzed HGM samples using MinPath/MetaCyc approach, thus we did not compare them with predicted phenotype abundances for vitamin B12.