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. 2019 May 29;10:1215. doi: 10.3389/fimmu.2019.01215

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Copyright © 2019 Maia, Cruz, de Oliveira, Samary, Fernandes, Trivelin, Rocha, Gama de Abreu, Pelosi, Silva and Rocco.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Expression of biological markers. Real-time polymerase chain reaction analysis of monocyte chemoattractant protein-1 (MCP-1) and tumor necrosis factor (TNF)-α from Control and Obese animals treated with saline (SAL) or endotoxin (ALI) intratracheally. Left panel: Blood neutrophils. Right panel: BALF neutrophils. Relative gene expression was calculated as the difference in average gene expression levels compared with the reference gene 36β4 (acidic ribosomal phosphoprotein P0) and expressed as fold change relative to Control-SAL. Boxes show the interquartile range (25–75%), whiskers denote the range (minimum–maximum), and horizontal lines represent the median in nine animals/group.