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. 2019 Jun 5;10:534. doi: 10.3389/fgene.2019.00534

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Copyright © 2019 Barhoumi, Nashabat, Alghanem, Alhallaj, Boudjelal, Umair, Alarifi, Alfares, Al Mohrij and Alfadhel.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Identification of differentially expressed genes in PBMCs obtained from patients with the DLL1 defect or controls. Cell pellets harvested from PBMCs of patients or controls were used for RNA extraction and the subsequent qRT-PCR analysis. Upregulated (A) and downregulated (B) genes in patients versus controls. The ΔΔCt analysis was performed using the SDS 5.4 (Applied Biosystems). GAPDH was used as a reference control for all analyses. Data are presented as means ± SEM. Data were analyzed using the paired t-test. ^∗P < 0.05 vs. control.