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The Golgi-Cox impregnation and cryoprotection. (A) The rat brain was cut in a sagittal manner and immersed in the Golgi-Cox solution in the dark. (B,C) Tissue was transferred into fresh solution on the 2nd day, and kept at least for 2 weeks. (D,E) After the impregnation step, tissues were shifted to the cryoprotection solution in the dark and (F) the solution was replaced on the 2nd day.
