Figure 2.

Brain sectioning. (A–C) The impregnated tissue was mounted with distilled water (DW) in mold and frozen in the cryostat. (D–F) After being sectioned, the slice was transferred by a retriever onto a gelatin-coated slide with few drops of sucrose solution on it. (G,H) We used a fine paint brush to adjust the position of the brain section and carefully covered it with a piece of lens cleaning tissue so that the section did not moved. (I,J) A filter paper was applied above the lens cleaning tissue to absorb the excess solution, and we pressed the paper to make sure any excessive solution was absorbed and no air bubbles were left between the section and the slides. (K,L) Move the filter paper and lens cleaning tissue away and be careful not to alter the position of the sections. Put the sections in a rack at room temperature overnight until they are fully dried.