Figure 2.

ATL-1 treatment decreases CD115⁺ LY6C^hi cell population in bone marrow of tumor-bearing mice. C57/BL6 mice were inoculated with B16/F10 tumor cells. After 14 days, ATL-1 (1 μg/animal) or vehicle (20 μL ethanol) was administered. On 21st day, the animals were sacrificed, the femurs and tibias dissected, and the bone marrow cells isolated for further analysis by flow cytometry. (A) Representative dot plot used to quantify the percentage of CD115⁺/LY6C^hi and CD115⁺/LY6C^low populations within the selected gate. (B) CD115 ⁺ LY6C^hi (n = 7); (C) CD115 ⁺ LY6C^low (n = 7). Data are expressed as mean ± SEM. *p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001 by unpaired t-test or ANOVA test, followed by Bonferroni test.