Figure 4.

ATL-1 treatment decreases blood circulating CD115⁺LY6C^hi monocyte population of tumor-bearing mice. C57/BL6 mice were inoculated with B16/F10 tumor cells. After 14 days, ATL-1 (1 μg/animal) or vehicle (20 μL ethanol) was administered. On 21st day, the animals were anesthetized, and blood was withdrawn by cardiac puncture. Thereafter, monocytes were isolated from the blood for further labeling with specific antibodies for flow cytometry analysis. (A) Representative dot plot used to quantify the percentage of CD115⁺/LY6C^hi and CD115⁺/LY6C^low populations within the selected gate. (B) CD115⁺ LY6C^hi (n = 5–6); (C) CD115⁺ LY6C^low (n = 5–6). Data are expressed as mean ± SEM. *p < 0.05; **p < 0.01 by unpaired t-test or ANOVA test, followed by Bonferroni test.