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. 2019 Jun 19;9:540. doi: 10.3389/fonc.2019.00540

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Copyright © 2019 de-Brito, da-Costa, Simões and Barja-Fidalgo.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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ATL-1 treatment decreases M2 markers and increases M1 markers expression of TAMs in vivo. C57/BL6 mice were inoculated with B16/F10 tumor cells. After 14 days, ATL-1 (1 μg/animal) or vehicle (20 μL ethanol) was administered i.v. After additional 7 days, animals were sacrificed, the tumors were dissected, and TAMs isolated for further analysis by flow cytometry, western blotting and RTqPCR. (A) F480⁺/ CD206⁺ cells (n = 9); (B) Arginase-1 gene expression (n = 6); (C) iNOS gene expression (n = 6) and (D) arginase-1 protein expression (n = 4). Gene expression is relative to the expression on 14th day. GAPDH or actin were used as housekeeping genes. Data are expressed as mean ± SEM. *p < 0.05; ***p < 0.001 by unpaired t-test.