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. 2019 Jun 19;9:540. doi: 10.3389/fonc.2019.00540

Figure 7.

Figure 7

ATL-1 treatment diminished tumor-macrophage infiltration and is able to improve the survival of tumor-bearing mice. C57/BL6 mice were inoculated with B16/F10 tumor cells. After 14 days, ATL-1 (1 μg/animal) or vehicle (20 μL ethanol) was administered. On 21st day, some animals were euthanized, and the tumor extracted. Some animals were left in the cage until day 25 for the survival analysis. Mphi (macrophages) were isolated and counted using a Neubauer chamber. (A) The graph represents the number of tumor-infiltrating macrophages (n = 4–5). Data are expressed as mean ± SEM. *p < 0.05 by unpaired t-test; (B) Survival curve (n = 6), the survival was recorded according to Kaplan–Meier analysis and analyzed by log-rank (Mantel–Cox) test; (C) Representative pictures and quantitative analysis (weight) of tumors developed in mice treated (lower row) and non-treated (upper row) with ATL-1. Data are expressed as mean ± SEM. *p < 0.05 by unpaired t-test.