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. 2019 Jun 17;7:1923. Originally published 2018 Dec 11. [Version 2] doi: 10.12688/f1000research.17268.2

Table 5. Reported Immunohistochemical Staining Results Identified in this Systematic Review.

IHC Staining
Target 		Epidermis Dermis Hair Follicles Sinus Tracts Subcutis Apocrine/
Eccrine
Glands 		Study
Reference
Suprabasal
Staining 		Basal
Staining 		Dermal
Staining 		Infundibular
Staining 		ORS
Staining 		Type 1 Type 2 Type 3
Type A Type B Type C
CD1a + 30
++ + 40
+++ 12
CD3 ++ ++ ++ 30
+ + + 40
33
CD4 ++ 12
Interfollicular and perifollicular 33
+ + 30
32
CD8 + 12
Epidermotropism Interfollicular and perifollicular 33
+ + 30
CD11c +++ 12
+++ 40
CD14 12
CD15 + 34
CD19 - + 40
CD20 +++ 30
+++ 12
CD29 + + + + (NOS) 35
CD32 32
CD56 30
- + 40
CD68 Deep> Perifollicular ++ + 30
Interfollicular and perifollicular 33
+ +++ 40
32
CD79 Interfollicular and perifollicular 33
CD138 Mild infiltrate 12
CD206 + +++ 40
CD207 +++ * + 40
CD209 ++ ++++ 40
Cytokeratins
AE1 Single K 36
AE3 Single K 36
PKC26 Single K 36
Factor XIIIa DC + 12
+ + 30
K1 Present in acanthotic epidermis + - + + - - 37
K2e ++ - - - 38
K4 - - - - 38
K5 + + + + 38
K6 - + + + 38
K5/6 § ++++ 30
K7 - - - + 7
K8 - - - - 38
K9 - - - - 38
K10 - 36
Present in acanthotic Epidermis + - + + - - 37
++ + ++ - - 38
K13 - + + - 38
K13+15+16 § + + + + + 38
K14 Highly positive in acanthotic
epidermis		 + + + + ++ Sebaceous
Duct and
Gland +		 37
+ + + 38
K15 Stained
apocrine
glands		 34
K16 Weakly positive in acanthotic
epidermis		 - + - + + - 37
K17 Weakly positive in acanthotic
epidermis		 - + - + + - 37
K18 38
K19 + NOS + 36
Weakly positive in acanthotic
epidermis		 - + - - - - 37
- - ++ 38
K20 - - - 38
Ki67 + ++ ++ ++ 38
ER - + 39
AR - NC 39
GCDFP-15 Apocrine
glands		 34
S100 Eccrine
glands		 34
Lysozyme Vulval
cases only		 34
↓ in scarred cases 14
HMB45 - Negative
all cases		 34
TLR2 ++ ++++ 40
↓ 41
TLR3 ↓ 41
TLR4 ↓ 41
TLR7 ↓ 41
TLR9 ↓ 41
ICAM-1 ↓ 41
TGF-β ↓ 41
IGF-1 ↓ 41
RNase7 +++ 42
MMP2 +++/++++ +++/++++ + +++ + NOS 13
MMP8 (Neutrophils) +++ (Neutrophils) 43
Cholera Toxin Slopes of papillae suprabasal
epidermis,		 hair follicles + NOS 35
Desmoplakin
1		 ++ ++ ++ + 38
Desmoplakin
2		 ++ ++ ++ + 38
Plakoglobin ++ ++ ++ + 38
Plakophilin 1 ++ ++ ++ + 38
Plakophilin 2 - - - 38
Desmoglein 1 ++ + ++ ++ - 38
Desmoglein 2 + + ++ ++ 38
Desmoglein 3 ++ ++ ++ + 38
Desmocollin 1 ++ + ++ - - 38
Desmocollin 2 ++ + ++ ++ + 38
Desmocollin 3 ++ ++ ++ + 38
hBD2 ↓ Negative in
12/14 		1
↓ 41
hBD3 ++ (suprabasal) - ++ 14
+++ + 42
hBD4 ↓ 41
TNF- α ++/+++ (macrophage/
lymphocytes)		 ++/+++ +++ 13
++ ++ + NC ↓ 14
IL-6 ↓ 41
IL-10 ↓ 41
IL-12 ++++ 32
IL-23 ++++ 32
IL-17 Diffuse 30
+++ 32
IL-32 ++ +++ 4
IL-36 + +++ 6
Suprabasal +++ 5
Caspase1 ++ 30
NLRP3 ++ 30
MIF ++ ++ 14
S100A7 ++ +++ ++ 14
++ 41
LL-37 ++ ++ NC 14
++ +++ ++ 29
α -MSH ++ NC 14

Key: + to ++++ = Degree of positive staining, - = reported negative staining, NC= No Change; ↓ Decreased, NOS= Not Otherwise Specified, *= Statistically significant result compared with healthy controls, §=Pan Cytokeratin Stain, DC= Dendritic Cells, Single K= Single keratinocytes,