Table 6. Immunohistochemistry stains/antibodies used in included reviews.
| Target | Details | Study
Reference |
|---|---|---|
| CD1a | CloneO10; Dako Cytomartion | 30 |
| CloneO10; Dako Cytomartion | 40 | |
| O10 1:20 Immmunotech, Prague, Czech Republic | 12 | |
| CD3 | clone F7.2.38; Dako) | 30 |
| Polyclonal rabbit anti-human CD-3 dilution 1:25; Dako Cytomation Denmark A ⁄ S, Glostrup, Denmark), | 33 | |
| Polyclonal 1:150 Dako, Glostrup, Denmark | 12 | |
| Clone PC3/188A; DakoCytomation, Glostrup, Denmark | 40 | |
| CD4 | 4B12 1:160 Monosan Uden The Netherlands | 12 |
| monoclonal mouse anti-human CD-4 dilution 1:10; Vision Biosystems Novocastra, Newcastle, UK | 33 | |
| clone 4B12; Dako | 30 | |
| MT310 Dako | 32 | |
| CD8 | C9/144B 1:100 Dako | 12 |
| monoclonal mouse anti-human CD-8 dilution 1:50; Dako Cytomation Denmark A ⁄ S | 33 | |
| clone C8/144B; Dako | 30 | |
| CD11c | 5D11 1:60 Novocastra Newcastle Upon Tyne, UK | 12 |
| Clone KB90 DakoCytomation | 40 | |
| CD14 | MY4 1:100 Novocastra Newcastle Upon Tyne, UK | 12 |
| CD15 | Not Reported | 34 |
| CD19 | Clone HD37; DakoCytomation | 40 |
| CD20 | clone L26 (1,4); Dako | 30 |
| L 26 1:400 Dako | 12 | |
| CD29 | fluorescein-tagged B-subunit of cholera toxin (CTx-FITC) + CyChrome (Pharmingen BD Biosciences, Franklin Lakes, NJ, USA) | 35 |
| CD32 | KB61 Dako | 32 |
| CD56 | clone 123C3; Dako | 30 |
| Clone MOC-1; DakoCyotmation | 40 | |
| monoclonal mouse anti-human CD-56 1:50; Dako Cytomation Denmark A ⁄ S), | 33 | |
| 123C3.D5 1:25 Thermo Fisher Scientific Altrincham UK | 12 | |
| CD68 | Clone PG- M1; Dako) | 30 |
| monoclonal mouse anti-human CD-68 dilution 1:50; Dako Cytomation Denmark A ⁄ S) | 33 | |
| Clone EBM11; Dako Cytomation | 40 | |
| KP1 1:160 Dako | 12 | |
| EBM11 Dako | 32 | |
| CD79a | monoclonal mouse anti-human CD-79 dilution 1:25; Dako Cytomation Denmark A ⁄ S) | 33 |
| JCB117 1:100 Dako | 12 | |
| CD138 | B-A38 1:25 IQ Products Groningen, The Netherlands | 12 |
| CD206 | Clone 19.2; BD Biosciences Pharmingen | 40 |
| CD207 | Clone DCGM4; Immunotech, Marseilles, France | 40 |
| CD209 | Clone DCN46; BD Biosciences Pharmingen, San Diego Ca USA | 40 |
| Cytokeratins | ||
| Pankeratin | AE1/AE3/PKC26; Ventana Medical Systems SA, Illkirch, Cedex, France | 36 |
| AE1/AE3 1:200 Thermo Fisher Scientific | 12 | |
| Factor XIIIa | AC-1A1 1:200 Thermo Fisher Scientific | 12 |
| clone E980.1; Leica Biosystems Newcastle, Newcastle upon Tyne, U.K.) | 30 | |
| K1 | 34 Beta B4 Novo Castra Laboratories Ltd, Newcastle- upon-Tyne, UK) | 37 |
| K2e | Ks2́ 342́ 7.1 against CK 2e (Dr L.Langbein, Heidelberg, Germany), | 38 |
| K4 | 6B10 against CK 4, | 38 |
| K5 | AE 14 against CK 5, | 38 |
| K6 | Ks6.KA12 against CK 6, | 38 |
| K5/6 | clone M7237; Dako | 30 |
| CK7 | OV-TL 12/30 and Ks7 ´18 against CK 7, | 38 |
| K8 | CAM 5 ´2 against CK 8, | 38 |
| K9 | HK9TY1 (guinea-pig polyclonal) against CK 9 (Dr L.Langbein) | 38 |
| K10 | Not Reported | 36 |
| LHP1 Novo Castra Laboratories Ltd, Newcastle- upon-Tyne, UK) | 37 | |
| MoAbs K8́ 60 and DE-K10 against CK 10, | 38 | |
| K13 | Ks13 ´1 against CK 13, | 38 |
| K13+15+16 | Ks8 ´12 against CK 13 15 16, | 38 |
| K14 | LL001 Novo Castra Laboratories Ltd, Newcastle- upon-Tyne, UK) | 37 |
| LL001 against CK 14, | 38 | |
| K15 | Not Reported | 34 |
| K16 | LL025 Novo Castra Laboratories Ltd, Newcastle- upon-Tyne, UK) | 37 |
| K17 | E3 Novo Castra Laboratories Ltd, Newcastle- upon-Tyne, UK) | 37 |
| Ks17.E3 against CK 17, | 38 | |
| K19 | Not reported | 36 |
| B170 Novo Castra Laboratories Ltd, Newcastle- upon-Tyne, UK) | 37 | |
| Ks19́1 against CK 19, | 38 | |
| K20 | IT-Ks20́10 against CK 20 | 38 |
| Ki67 | MIB 1 against Ki-67 | 38 |
| MIB1 1:100 Dako | 12 | |
| ER | ER (Thermo Scientific; pretreatment EDTA, pH 9.0, dilution 1:80). | 39 |
| AR | AR (Santa Cruz; pretreatment citrate, pH 6.0, dilution 1:100) | 39 |
| GCDFP-15 | Not Reported | 34 |
| S100 | Not Reported | 34 |
| Lysozyme | Not Reported | 34 |
| A0099 pAbG 1:100 rabbit antihuman Dako Corporation | 14 | |
| HMB45 | Not Reported | 34 |
| TLR2 | Clone TL2.3; Alexis Corp. San Diego Ca USA | 40 |
| Santa Cruz Biotechnology, Inc, Santa Cruz, California | 41 | |
| TLR3 | Santa Cruz Biotechnology, Inc, Santa Cruz, California | 41 |
| TLR4 | Santa Cruz Biotechnology, Inc, Santa Cruz, California | 41 |
| TLR7 | Santa Cruz Biotechnology, Inc, Santa Cruz, California | 41 |
| TLR9 | Santa Cruz Biotechnology, Inc, Santa Cruz, California | 41 |
| ICAM-1 | Beckman Coulter, Inc, Brea, California | 41 |
| TGF-β | AbD Serotec | 41 |
| IGF-1 | R&D Systems, Inc, Lille, France | 41 |
| RNase7 | Dako | 42 |
| MMP2 | MMP-2 (cat no. AF902, LOT DUB034081, obtained from goat, 1:100 dilution, R&D Systems | 13 |
| MMP8 | Dako | 43 |
| Cholera Toxin | fluorescein-tagged B-subunit of cholera toxin (CTx-FITC) + CyChrome (Pharmingen BD Biosciences, Franklin Lakes, NJ, USA) | 35 |
| Desmoplakin 1 | DP 1 2±2́15 and DP 1±2́17 against DP I II, | 38 |
| Desmoplakin 2 | DP 1 2±2́15 and DP 1±2́17 against DP I II, | 38 |
| Plakoglobin | PG 5́1 and PG 11E4 (Dr M.J.Wheelock, Toledo, OH, U.S.A.) against PG, | 38 |
| Plakophilin 1 | PP1-9E7 and PP1-5C2 against PP 1, | 38 |
| Plakophilin 2 | PP2- 150 against PP 2, | 38 |
| Desmoglein 1 | Dsg1E-P124 and Dsg1E-P23 against Dsg1, | 38 |
| Desmoglein 2 | Dsg2E-G129 and Dsg2E- G96 against Dsg2, | 38 |
| Desmoglein 3 | Dsg3-G194 and 5G11 against Dsg3, | 38 |
| Desmocollin 1 | Dsc1-U100 against Dsc1, | 38 |
| Desmocollin 2 | DC-Rab 36 (rabbit polyclonal) against Dsc2, | 38 |
| Desmocollin 3 | MoAb Dsc3-U114 against Dsc3, | 38 |
| hBD2 | Human beta-defensin 2 (cat no. AF 2758, LOT VJU015051, obtained from goat, 1:100 dilution, R&D Systems, Germany) | 13 |
| Abcam, San Francisco, California | 41 | |
| hBD3 | 1:400 rabbit antihuman Donated by Prof Schroders Labor Kiel germany | 14 |
| 1:1000 rabbit ani-human PeproTech, Rocky Hill, N J | 42 | |
| hBD4 | Abcam, San Francisco, California | 41 |
| TNF-α | TNF-α (code ab 6671, obtained from rabbit, 1:100 dilution, Abcam, Cambridge, UK | 13 |
| 559071 mAbG 1:10 mouse antihuman R&D Systems | 14 | |
| IL-6 | AbD Serotec, Oxford, England | 41 |
| IL-10 | R&D Systems, Inc, Minneapolis, Minnesota | 41 |
| IL-12 | IL-12p7024945 R&D Systems | 32 |
| IL-23 | IL23p19 HLT2736 Biolegend | 32 |
| IL-17 | clone AF-317-NA; R&D Systems, Wiesbaden Germany | 30 |
| Polyclonal R& D Systems | 32 | |
| IL-32 | NBP-76684, Novus (Littleton, CO, U.S.A.) | 4 |
| IL-36 | rabbit polyclonal anti-IL-36a (C-terminal; ab180909), rabbit polyclonal anti- IL-36b (C-terminal; ab180890) and mouse
monoclonal anti-IL-36c (ab156783; all from Abcam, Cambridge, U.K. |
6 |
| AF 1078,1099,2320,1275 RnD | 5 | |
| Caspase1 | clone 14F468; Imgenex/Novus Biologicals, Littleton, CO, U.S.A.) | 30 |
| NLRP3 | clone Ab17267; Abcam, Cambridge, U.K. | 30 |
| MIF | MAB289 mAbG 1:100 mouse antihuman | 14 |
| S100A7 | HL15-4 mAbG 1: 20,000 mouse antihuman Donated by Prof Schroders Labor Kiel germany | 14 |
| LL37/
Cathelicidin |
Ab64892 pAbG 1:1000 rabbit antihuman Abcam | 14 |
| Rabbit anti-human LL-37 [Abcam, Cambridge, UK | 29 | |
| α-MSH | M0939 1:500 Rabbit Anithuman Sigma | 14 |
| PROGEN Biotechnik GmbH, Heidelberg, Germany | 41 | |
| Tryptase | AA1 1:800 Dako | 12 |
| clone AA1; Dako | 30 |