Table 1.
Gene specific PCR and overgo primers.
| Identified | PCR product | |||
|---|---|---|---|---|
| Gene symbol | BAC clones | PCR primer 5′-3′ | size (bp) | Overgo primer sequence 5′-3′ |
| ALX3 | 115I10 | F: TATGTCTCCGTACTCCCACTCTCR: GGAGACTTATAGTCGTCATCTGG | 161 | F: GCTCTAGGGGGCCACAGCTTTGAGR: CGTCATCTGGGGAGGGCTCAAAGC |
| R: GGAGACTTATAGTCGTCATCTGG | R: CGTCATCTGGGGAGGGCTCAAAGC | |||
| COL1A1 | 198E13 | F: CCATTGGTAGTGTTGGTGCT | 365 | F: GCCCTGTTGGCAAAGAAGGCAGCA |
| 204B18 | R: AGGGAAGCCTCTTTCTCCTC | R: TCACCACGAGGACCTTTGCTGCCT | ||
| 264O11 | ||||
| 271L20 | ||||
| 295O17 | ||||
| CTNNB1 | 129B09 | F: ATCCCAGCTATCGTTCTTTTCA | 300 | F: CACTCCGGTGGATACGGACAGGAT |
| 150A21 | R: CCTACCAACCCAAGTCTTTCTG | R: GGTCCATACCCAAGGCATCCTGTC | ||
| DAB2IP | 101B06 | F: TACTGAGAACGGCGAGTTCA | 107 | F: GAACGGCGAGTTCAGAAACAGCAGCAA |
| R: AAAGCTCAGCCTCTCTCTCG | R: CGTGCCTGGGACACTTGAATTGCTGCT | |||
| KRT15 | 263E22 | F: GGCAAAGTCCGCATCAATGTT | 218 | F: TGGCCAGAGGGGCCAGAAGGGCAAA |
| 268A9 | R: ATGCCAAGCAGCCAACTAGG | R: CCCCTCTGGGTCTAGAGTTTGCCCT | ||
| 274A22 | ||||
| KRTAP13-1 | 336H05 | F: GCAAAGGCTACTTCCTGGTCTA | 109 | F: TCCAGAAGCTGTGGGTCCAGTGG |
| 368J2 | R: ATTGGATGGCAGGATCCACAG | R: TCCAGAACCCAGAGATCCACTGGA | ||
| 408J12 | ||||
| 413H10 | ||||
| NCOA6 | 34F15 | F: CCCAAGATTTTCTAAAGACAGGAA | 151 | F: CAGCTGTGTTTACAACTCCTCCAGCCAAG |
| 46J23 | R: CTGGTCAGTATGGGCTTATCTCTT | R: CTGGTCAGTATGGGCTTATCTCTTGGCTG | ||
| 59N23 | ||||
| 86O24 | ||||
| SOX9 | 13O23 | F: AAATGCTCTTATTTTTCCAACAGC | 220 | F: GTGTTATGGGATCAGTTTGGGGGGTTA |
| 30B6 | R: AATCACAAAGCCTGAGGAATTAAG | R: CTGAGGAATTAAGCAAAGCTAACCCCC | ||
| 32I21 | ||||
| 58P4 | ||||
| 68P18 | ||||
| 115A15 | ||||
| 122H18 | ||||
| 169O5 | ||||
| 172F10 | ||||
| 186L14 | ||||
| 202F10 | ||||
| 231H17 | ||||
| 249C14 | ||||
| 279H10 | ||||
| 297J24 | ||||
| 306O5 | ||||
| TNFSF12 | 169O5 | F: GACCTGAATCCCCAGACAGA | 94 | F: AGCCAGGACACCGTGTCTTTCCTG |
| 133N9 | R: GTGGTTTCCGGCCTTTAGGT | R: GAGGCCGAACCAGTTTCAGGAAAG | ||
| 172F10 | ||||
| ZIC1 | 127I17 | F: AGTCCGCGTTCAGAGCACTAT | 192 | F: GCGCCGGCGCTTTCTTCCGCTACATG |
| 135I16 | R: GAAAGTTTTGTTGCACGACTTTTT | R: CTGTTTGATGGGCTGGCGCATGTAGC | ||
| ZIC5 | 211H22 | F: GCAAACTTTCTGCAAGTGCAAC | 199 | F: AGGGGGCACGAAGCGAAAGCGAAG |
| 224A3 | R: GGAAGCCTGTCATATTCTGAAAC | R: CTGTGCTCACTGACGCCTTCGCTT |
BAC numbers in bold denote those that were used for gene chromosomal localization by FISH.