Fig. 1.

Clodronate-liposomes deplete CD169-expressing macrophages within the BM.

KaLwRij mice were treated once with PBS-lip, zol, or clo-lip. Total BM was isolated from long bones after 24 hours (A, B) or as indicated (C); stained with Cd11b, F4/80, and CD169 fluorescently conjugated antibodies; and analyzed by flow cytometry. (A) Cd11b⁺F4/80⁺CD169⁻ monocyte/macrophage population (black; n.s, P = .694) and Cd11b⁺F4/80⁺CD169⁺ mature macrophages (gray; ****P < .0001) expressed as a percentage of total viable cells. (B) Representative FACS plots of Cd11b⁺F4/80⁺CD169⁺ mature macrophages. (C) Recovery of Cd11b⁺F4/80⁺CD169⁺ mature macrophages following a single injection of clo-lip over a 28-day period. n = 3/group. Graphs show mean ± SEM, *P < .05, **P < .01, ****P < .0001, 2-way ANOVA with Tukey's multiple comparisons test.